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目的筛选与涎腺腺样囊性癌转移相关的候选基因,并对其中的候选基因进行初步的验证。方法用限制片段差异显示PCR技术(restriction fragments differential display PCR,RFDD-PCR)建立涎腺腺样囊性癌高、低转移细胞株(ACC-M、ACC-2)的表达谱。对两个表达谱的片段进行比较,通过生物信息学的分析,初步筛选出候选基因。用半定量逆转录PCR技术对筛选出的基因进行初步验证。结果 RFDD-PCR方法共获得5420个基因片段,其中包含12个基质金属蛋白酶(matrix metalloproteinase ,MMP)基因。半定量逆转录PCR方法发现MMP2、MMP7、MMP9、MMP14、MMP15、MMP24在ACC-M和ACC-2中的表达存在明显差异。结论构建了ACC-M和ACC-2细胞株的表达谱,为寻找目的基因奠定了基础。发现MMP2、MMP7、MMP9和MMP15与涎腺腺样囊性癌的发生、发展、转移有关,不同肿瘤细胞的转移能力可能与不同的MMPs家族基因相关。
Objective To screen candidate genes related to the metastasis of salivary adenoid cystic carcinoma and to validate the candidate genes. Methods The expression profiles of ACC-2 and ACC-2 in salivary adenoid cystic carcinoma were established by restriction fragment differential PCR (RFDD-PCR). The fragments of the two expression profiles were compared, and bioinformatics analysis was used to screen candidate genes initially. Semi-quantitative RT-PCR was used to validate the selected genes. Results A total of 5420 gene fragments were obtained by RFDD-PCR, including 12 matrix metalloproteinase (MMP) genes. Semi-quantitative RT-PCR showed that the expression of MMP2, MMP7, MMP9, MMP14, MMP15 and MMP24 in ACC-M and ACC-2 were significantly different. Conclusion The expression profiles of ACC-M and ACC-2 cell lines were constructed, which laid the foundation for finding the target gene. Found that MMP2, MMP7, MMP9 and MMP15 and salivary adenoid cystic carcinoma of the occurrence, development, metastasis, different tumor cell metastasis may be related to different MMPs family of genes.