实验室条件下采用生长速率法测定化合物N-(4-甲基-2-氨基苯并噻唑)α-氨基-α-(3-三氟甲基苯基)-O,O-二(2-烷氧基乙基)亚膦酸酯对小麦赤霉病原菌(Fusarium graminearum)的离体抑制效果,并初步研究了其抑制小麦赤霉病原菌作用机制。实验结果表明,该化合物对小麦赤霉病原菌的EC_(50)为46.05μg/mL,当化合物浓度为50μg/mL时,对该病原菌的抑制率就达到了60.5%。以浓度为250μg/mL的该供试化合物处理小麦赤霉病原菌菌丝24 h后,其细胞膜通透性增强,菌体内还原糖、几丁糖和可溶性蛋白含量及几丁质酶活性在短时间内均出现先升高然后下降的趋势。 The compound N- (4-methyl-2-aminobenzothiazole) α-amino-α- (3-trifluoromethylphenyl) -O, O- Alkoxyethyl) phosphonite against Fusarium graminearum in vitro and its mechanism of inhibiting the pathogen of Fusarium graminearum was preliminarily studied. The results showed that the compound had an EC50 of 46.05μg / mL against the pathogen of Gibberella zeae, and reached 60.5% inhibition rate when the compound concentration was 50μg / mL. The concentration of 250μg / mL of the test compound treatment Gibberella zeae pathogen mycelia 24 h, the enhanced cell membrane permeability, bacterial body reducing sugar, chitosan and soluble protein content and chitinase activity in a short time Within the first appeared to rise and then decline.