中间纤维、微管与微丝是胞质中的纤维状细胞器,对维持细胞外形、细胞器在细胞内的位置、调节细胞内与细胞间的运动等具有重要作用。角蛋白是直径为8～12nm的中间纤维。本文应用免疫荧光法研究了培养的正常人食管上皮,食管癌细胞与诱导分化前后食管癌细胞中间纤维角蛋白纤维的表达及其与张力原纤维的变化,以探讨角蛋白在肿瘤细胞分化与在肿瘤细胞生物学特性中的意义。 Intermediate fibers, microtubules and microfilaments are fibrous organelles in the cytoplasm, which play an important role in maintaining the shape of cells, the location of organelles in cells, and the intracellular and intracellular movement. Keratin is an intermediate fiber with a diameter of 8 to 12 nm. In this paper, immunofluorescence method to study the normal esophageal epithelial cells and esophageal cancer cells before and after induction of differentiation of esophageal cancer cells in the intermediate fiber keratin fibers and fibrillation of the changes in order to investigate keratinocyte differentiation and Significance of tumor cell biology.