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以球内叶橘红色的大白菜和外叶紫色的普通白菜(小白菜)为亲本进行杂交、自交和回交,研究大白菜橘红色、紫色2对基因的遗传规律。结果表明:球内叶橘红色的大白菜和外叶紫色的普通白菜杂交,F1植株颜色为非橘红心-紫色;在F2群体中出现4种基因型,非橘红心-紫色、非橘红心-非紫色、橘红心-紫色、橘红心-非紫色,比例接近9:3:3:1,其中获得橘红心-紫色基因型ororPr-的概率为18.75%,获得纯合橘红心-紫色基因型ororPrPr的概率为6.25%;在BC1群体中,4种基因型之比接近1:1:1:1;BC2群体中,全部植株颜色为非橘红心-紫色。x2检测结果进一步证明:球内叶橘红色的大白菜和外叶紫色的普通白菜杂交后代球内叶橘红色和外叶紫色为非连锁、互为独立遗传、自由组合的2对基因,二者分别位于不同的染色体上。球内叶橘红色基因or已定位在A基因组1号染色体末端,据此推断,来源于外叶紫色的普通白菜的紫色基因不在1号染色体上,而是在A基因组其他染色体上。
The inheritance of two genes of mandarin orange and purple in Chinese cabbage was studied by crossing the orange-red cabbage and the outer purple cabbage (cabbage). The results showed that the F1 hybrids were orange-red heart-purple in F1 hybrids and non-orange-red-purple red-orange-red hearts in F2 population. Non-purple, orange heart - purple, orange heart - non-purple, the ratio close to 9: 3: 3: 1, wherein the probability of obtaining orange heart-purple genotype ororPr- is 18.75%, obtaining homozygous red heart-purple genotype ororPrPr In the BC1 population, the ratio of the four genotypes was close to 1: 1: 1: 1 in the BC1 population; and in the BC2 population, the color of all the plants was non-orange red heart-purple. x2 test results further prove that: the inner leaves of the orange-red cabbage and the outer leaves of purple cabbage hybrid offspring of the inner leaves of the orange leaves and the outer leaves of purple non-linked, independent of each other genetic, free combination of the two pairs of genes, both Respectively located on different chromosomes. The intracochlear reddish orange gene, Or, has been located at the end of chromosome 1 of genome A, suggesting that the purple gene of common cabbage originating from outer purple is not on chromosome 1, but on other chromosomes of genome A.