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The coding propertics of each mRNA synthesized in vitro of CPV have been examined. The mRNAs were separated by polyacrylamide gel electrophoresis and isolated from the gel slices. The individual mRNA was then translated into the wheat germ cell-free protein synthesizing system. The products of translation were analyzed by the immuno-reaction with the ~(125)I-IgG of each of the five electrophoretic protein components of CPV. The coding assignments were deduced on the basis of the immuuo-reaction as follows: mRNA_1, mRNA_3 and mRNA_9 encode group P_1 of viral proteins, mRNA_2 and mRNA_6 encode group P_2 and P_3, respectively, mRNA_7 and mRNA_6 encode group P_4 while mRNA_9 encode group P_5.There axe more than 10 polypeptides in the CPV virion as demonstrated by two-dimensional electrophoresis.
The coding properties of each mRNA synthesized in vitro of CPV have been examined. The mRNAs were separated by polyacrylamide gel electrophoresis and isolated from the gel slices. The individual mRNA was then translated into wheat germ cell-free protein synthesizing system. The products of translation were analyzed by the immuno-reaction with the ~ (125) I-IgG of each of the five electrophoretic protein components of CPV. The coding assignments were deduced on the basis of the immuuo-reaction as follows: mRNA_1, mRNA_3 and mRNA_9 encode group P_1 of viral proteins, mRNA_2 and mRNA_6 encode group P_2 and P_3, respectively, mRNA_7 and mRNA_6 encode group P_4 while mRNA_9 encode group P_5.There ax more than 10 polypeptides in the CPV virion as demonstrated by two-dimensional electrophoresis.