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鉴于培养恶性疟原虫的技术都有某一方面的缺点,本文对原来的烛缸法作了如下改进。RPMI内葡萄糖浓度自2g/l增至4g/l,主要缓冲剂TES为4mM/l,所用气体含5%氧、5%二氧化碳、90%氮。采用50ml无菌的带螺旋盖的塑料培养瓶,内盛5ml红细胞悬液。培养时松开瓶盖,置入盛有少量硫酸铜液的玻璃干燥器中,以不超过300mmHg的真空泵抽真空后,通入上述混合气体,使内
In view of the culture of Plasmodium falciparum have some shortcomings, this article on the original candle cylinder made the following improvements. The glucose concentration in RPMI increased from 2 g / l to 4 g / l, the main buffer TES was 4 mM / l and the gas used contained 5% oxygen, 5% carbon dioxide and 90% nitrogen. Using 50ml sterile plastic flask with a screw cap, filled with 5ml of red blood cell suspension. When the bottle is uncovered, the bottle is placed in a glass drier containing a small amount of copper sulfate solution. After evacuating with a vacuum pump of not more than 300 mmHg, the mixed gas is introduced into the inner cylinder