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本研究利用实时荧光定量PCR技术监测施用生防菌前后土壤中粉红粘帚霉67-1和水稻纹枯病菌AG-IIA的数量,并调查发病情况,收割后测产,评估生防菌粉红粘帚霉67-1的田间防治效果。实时荧光定量检测结果表明,粉红粘帚霉67-1防治水稻纹枯病的效果随着用药浓度的增加而增强,各处理间差异极显著。用药后土壤中粉红粘帚霉67-1的含量在一定时间内随培养时间的延长逐渐升高,停止用药后逐渐减少。水稻纹枯病菌在清水对照组中没有显著变化,在处理组中水稻纹枯病菌的数量随时间降低。60 g/667 m2菌量的防效及产量与井冈霉素对照组差异不显著。防病保产结果与实时荧光定量结果基本一致,说明粉红粘帚霉67-1的孢子制剂对水稻纹枯病具有优良的田间防治效果,实时荧光定量PCR技术可以用于生物防治效果的田间评价。
In this study, real-time fluorescence quantitative PCR was used to monitor the amount of Gliadin 67-1 and Rhizoctonia solani AG-IIA in the soil before and after the biocontrol agent was applied. The incidence of the disease and the yield after harvest were assessed. Glitter 67-1 field control effect. The results of real-time fluorescence quantitative analysis showed that the effect of controlling the sheath blight of rice with Gliadin 67-1 increased with the increase of the drug concentration, and the difference among treatments was very significant. After treatment, the content of Gliadin 67-1 in soil increased gradually with the prolongation of culture time, and gradually decreased after stopping the treatment. Rhizoctonia solani did not change significantly in the fresh water control group, and the amount of R. solanacearum decreased with time in the treatment group. The control efficacy and yield of 60 g / 667 m2 were not significantly different from that of the control group of validamycin. The result of disease protection and protection was consistent with the result of real-time fluorescence quantitative analysis, indicating that the spores preparation of Gliadin grossedentum 67-1 had excellent field control effect on rice sheath blight and real-time fluorescence quantitative PCR technique could be used for field evaluation of biological control effect .