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取临床标本(性病门诊病人阴道分泌物)直接涂片染色镜检及用沙保氏琼脂平板分离培养、作涂片革兰染色镜检、出芽试验、厚膜孢子形成试验、糖发酵试验等鉴定菌种;小白鼠与家兔试验、荚膜肿胀试验、透射电镜等观察荚膜,观察白假丝酵母菌临床分离菌株(C1-1、C1-2、C1-3、C1-4)的荚膜结构并探讨其形成条件。结果显示:(1)C1-1、C1-2、C1-3、C1-4四株临床分离菌株均为革兰阳性、念珠状菌;能形成芽管、假菌丝、厚膜孢子;能发酵葡萄糖和麦芽糖产酸又产气;发酵蔗糖产酸不产气;不发酵乳糖。(2)在感染小白鼠及家兔体内均可形成荚膜,荚膜层的厚度可因不同环境而有显著差异(P<0·01或<0·05),边界明显;荚膜肿胀试验阳性。4株临床分离菌株均可被鉴定为具有荚膜的白假丝酵母菌。
Take the clinical specimens (STD clinic patients with vaginal secretions) direct smear microscopy and sand Shabu agar plate separation and culture for smear Gram staining microscopy, budding test, thick film spore formation test, sugar fermentation test identification The bacterial strains, mice and rabbits test, capsular swelling test, transmission electron microscopy and other observations capsule, observed Candida albicans clinically isolated strains (C1-1, C1-2, C1-3, C1-4) pods Membrane structure and its formation conditions. The results showed that: (1) The four clinically isolated isolates of C1-1, C1-2, C1-3 and C1-4 were Gram-positive, Candida-like bacteria; germ tube, pseudohyphae and thick-film spores were formed; Fermentation of glucose and maltose acid and gas; Fermented sucrose acid does not produce gas; Does not ferment lactose. (2) The capsule can be formed in the infected mice and rabbits. The thickness of the capsule can be significantly different due to different environments (P <0.01 or <0.05) with obvious boundary; capsule swelling test Positive. Four clinical isolates could be identified as Candida albicans.