论文部分内容阅读
目的明确重症肌无力(MG)患者胸腺组织CC趋化因子配体21(CCL21)的分布;通过CCL21基因转染MG患者细胞角蛋白8/18(CK8/18)阳性胸腺上皮细胞(TEC),探讨CCL21过表达对CK8/18阳性TEC中抗原提呈功能相关分子表达的影响。方法采用免疫组织化学检测CCL21、CK8/18阳性细胞在MG患者胸腺的表达及分布,采用实时荧光定量PCR(qRT-PCR)检测MG患者胸腺CCL21、CCL19及CC趋化因子受体7(CCR7)的mRNA水平;p CMV-CCL21质粒转染MG患者分离的CK8/18阳性TEC,qRT-PCR检测转染前后TEC功能性基因CD80、细胞间黏附分子1(ICAM-1)、CD86、HLA-DR、HLA-A的mRNA水平。结果 MG胸腺组织中CK8/18阳性细胞明显多于正常对照,且在皮质及髓质区均有分布,CCL21蛋白、CCR7mRNA的水平显著增高。p CMV-CCL21质粒转染TEC后,HLA-A、HLA-DR、ICAM和CD80 mRNA水平显著增加。结论 CCL21过表达,可引起MG患者CK8/18阳性胸腺上皮细胞抗原呈递功能相关基因的表达增高。
Objective To determine the distribution of CC chemokine ligand 21 (CCL21) in thymic tissue of patients with myasthenia gravis (MG). Cytokeratin 8/18 (CK8 / 18) positive thymic epithelial cells (TECs) To investigate the effect of CCL21 overexpression on the expression of antigen presenting function-related molecules in CK8 / 18-positive TECs. Methods The expression and distribution of CCL21 and CK8 / 18 positive cells in the thymus of patients with MG were detected by immunohistochemistry. CCR21, CCL19 and CCR7 were detected by qRT-PCR in MG patients. The expression of ICAM-1, CD86 and HLA-DR were detected by qRT-PCR before and after transfection of CK8 / 18 positive TECs transfected with p CMV-CCL21 plasmid. , HLA-A mRNA levels. Results The number of CK8 / 18 positive cells in MG thymus tissue was significantly more than that in normal controls, with distribution in cortex and medulla area. The levels of CCL21 protein and CCR7 mRNA were significantly increased. The levels of HLA-A, HLA-DR, ICAM and CD80 mRNA were significantly increased after transfected with p CMV-CCL21 plasmid. Conclusion Overexpression of CCL21 can cause the expression of antigen-presenting function-related genes in CK8 / 18 positive thymic epithelial cells of MG patients.