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以脱脂鼠角质层和提取角蛋白为模型,利用电化学交流阻抗谱、SEM、DSC、FTIR等手段,研究了低聚壳聚糖及氨基葡萄糖与角质层蛋白之间的相互作用.结果发现,氨基葡萄糖溶液处理不影响脱脂角质层膜的阻抗,而低聚壳聚糖溶液处理却可明显降低脱脂角质层膜的阻抗,阻抗值由3.79×106Ω·cm2降至8.379×105Ω·cm2.此外,壳聚糖溶液和氨基葡萄糖溶液都可明显破坏角蛋白表面致密均匀的结构,并使得角蛋白二级结构中α-螺旋结构含量减少,同时推动角蛋白α-螺旋结构向β-折叠结构和无规则卷曲结构转变.这些结果表明低聚壳聚糖与角质层蛋白相互作用,一方面可有效降低鼠角质层结构的紧密程度,另一方面可影响角蛋白的微结构,使其结构松散并出现微细孔道,从而为药物的经皮吸收与传送疏通了通道.
The interaction between oligochitosan, glucosamine and keratin layer proteins was studied by means of electrochemical impedance spectroscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR) The glucosamine solution treatment did not affect the degreasing stratum corneum membrane impedance, but the chitosan solution treatment can significantly reduce the degreasing stratum corneum membrane impedance, the impedance value from 3.79 × 106Ω · cm2 to 8.379 × 105Ω · cm2.In addition, Chitosan solution and glucosamine solution can obviously destroy the dense and uniform structure of keratin surface and decrease the content of α-helical structure in the keratin secondary structure, at the same time, promote the conversion of keratin α-helical structure to β-sheet structure and no Regular curly structure.These results indicate that the interaction between oligomeric chitosan and stratum corneum protein can effectively reduce the tightness of the stratum corneum structure and on the other hand, can affect the microstructure of keratin and make the structure loose and appear Micro-channel, thus drug percutaneous absorption and delivery to clear the channel.