为克隆和筛选肝癌特异表达基因.应用抑制消减杂交技术对肝癌及癌旁组织进行消减杂交,产物PCR扩增后,进行克隆.共得到1820个克隆,1776个克隆有100~800bp的插入片断,阳性率达98%,说明人肝癌组织特异表达cDNA消减文库构建成功,同时我们对cDNA克隆进行测序,证明这些克隆的功能是和肝癌的发生高度相关的.抑制消减杂交是克隆特异表达基因的有效方法. In order to clone and screen the hepatoma-specific genes, 1820 clones and 1776 clones with 100 ~ 800bp insertions were cloned by subtractive hybridization and subtractive hybridization, The positive rate of 98%, indicating that human hepatocellular carcinoma tissue-specific cDNA subtractive library was successfully constructed, and we cloned the cDNA sequence to prove that the function of these clones is highly correlated with the occurrence of liver cancer.Expression suppression subtractive hybridization is specifically expressed genes method.