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目的:探讨甲泼尼龙对百草枯(PQ)中毒大鼠肾脏损伤的保护作用。方法:30只健康SD大鼠随机分为对照组、PQ模型组及甲泼尼龙治疗组,每组10只。各组在第1、3、7d分别舌下静脉采血,检测血清肌酐(Cr)和血尿素氮(BUN)水平,第7天分离各组大鼠肾组织行HE染色后,比较各组大鼠肾脏的组织形态学差异,免疫组织化学SABC法染色观察和比较各组大鼠肾脏巨噬细胞移动抑制因子(MIF)的表达情况。结果:(1)与对照组相比,模型组第1、3、7天血清中Cr和BUN的水平均显著升高(P<0.05);与模型组相比,治疗组第3和7d血清中Cr和BUN水平均显著降低(P<0.05);(2)与对照组相比,模型组大鼠肾脏病理改变有充血水肿、炎症细胞浸润、变性坏死及结构紊乱等;与模型组比较,治疗组的病变明显减轻;(3)与对照组相比,模型组肾组织中MIF的阳性表达细胞数显著增加(P<0.05);与模型组相比,治疗组肾组织中MIF的阳性表达细胞数显著减少(P<0.05)。结论:甲泼尼龙可能通过减少MIF表达减轻百草枯对大鼠肾脏的损伤。
Objective: To investigate the protective effect of methylprednisolone on renal injury induced by paraquat (PQ) in rats. Methods: Thirty healthy SD rats were randomly divided into control group, PQ model group and methylprednisolone treatment group, with 10 rats in each group. Blood samples were collected from the sublingual vein on the 1st, 3rd and 7th day to detect serum creatinine (Cr) and blood urea nitrogen (BUN) levels. On the 7th day, the renal tissues of rats in each group were stained with HE. Kidney histological differences were observed by immunohistochemical SABC staining and compared the expression of renal macrophage migration inhibitory factor (MIF) in each group. Results: (1) Compared with the control group, the levels of serum Cr and BUN in the model group were significantly increased on the 1st, 3rd, 7th day (P <0.05). Compared with the model group, (P <0.05). (2) Compared with the control group, the renal pathological changes in rats in the model group were congestion and edema, infiltration of inflammatory cells, degeneration and necrosis, and structural disorders. Compared with the model group, (3) Compared with the control group, the number of MIF positive cells in the model group was significantly increased (P <0.05). Compared with the model group, the positive expression of MIF in the kidney tissue of the model group Cell number decreased significantly (P <0.05). CONCLUSION: Methylprednisolone may alleviate the injury of paraquat to rats’ kidneys by reducing the expression of MIF.