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目的 探讨丹参对急性脊髓损伤的防治作用。方法 以改良Allen’s法造成兔不完全性脊髓损伤的模型,硬膜下插管。随机分成丹参治疗组和对照组。术后按每天0.3ml/kg体重的总量分4次从硬膜下导管推入丹参注射液,对照组推入生理盐水。损伤后8、72h对脊髓损伤区进行过氧化物歧化酶(SOD)、丙二醛(MDA)、组织形态学观察、神经元凋亡、bcl-2等进行评价。结果 丹参组SOD含量高于对照组(P<0.01),MDA含量低于对照组(P<0.01)。细胞凋亡数目TUNEL法丹参组低于对照组(P<0.01),流式细胞术检测凋亡丹参组低于对照组(P<0.05)。bcl-2的表达丹参组高于对照组(P<0.05)。神经元及神经纤维变性、坏死轻于对照组。结论 丹参能改善损伤脊髓微循环,抑制和减轻脊髓损伤后的两种死亡方式坏死和凋亡。
Objective To explore the preventive and therapeutic effects of salvia miltiorrhiza on acute spinal cord injury. Methods The model of incomplete spinal cord injury in rabbits was modified by modified Allen’s method and subdural cannulation. Randomly divided into Salvia treatment group and control group. Postoperatively, the total amount of 0.3 ml/kg body weight was divided into four injections from the subdural catheter into the Danshen injection, and the control group was injected with saline. Superoxide dismutase (SOD), malondialdehyde (MDA), histomorphological observation, neuronal apoptosis, bcl-2, etc. were evaluated in spinal cord injury area at 8 and 72 hours after injury. Results The content of SOD in Danshen group was higher than that in control group (P<0.01), and the content of MDA in Danshen group was lower than that in control group (P<0.01). The number of apoptotic cells in the TUNEL method was lower than that in the control group (P<0.01). The flow cytometry detection of apoptosis in the Salvia miltiorrhiza group was lower than that in the control group (P<0.05). The expression of bcl-2 in Salvia miltiorrhiza group was higher than that in control group (P<0.05). The degeneration and necrosis of neurons and nerve fibers were lighter than those of the control group. Conclusion Salvia miltiorrhiza can improve microcirculation of injured spinal cord and inhibit and reduce necrosis and apoptosis of two death modes after spinal cord injury.