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[Objective] To detect the content of total flavonoids from Hedyotis scandens Roxb. [Method] With rutin as the reference substance, total flavonoids were extracted from H. scandens by ultrasonic-assisted extraction. Then, the content of total flavonoids was detected by UV Spectrophotometry. [Result] Rutin concentration and absorbancy had good linear relationship between 0.011 and 0.110 mg/mL. The regression equation was Y=18.015X+0.020; and correlation coefficient was R2=0.999 4. The relative percentage of total flavonoids in H. scandens was 1.609%. And the RSD in precision test was 1.10%. [Conclusion] This method was simple, reliable and could provide scientific references for the development and utilization of the medicinal value of H. scandens.
[Objective] To detect the content of total flavonoids from Hedyotis scandens Roxb. [Method] With rutin as the reference substance, total flavonoids were extracted from H. scandens by ultrasonic-assisted extraction. Then, the content of total flavonoids was detected by UV [Result] Rutin concentration and absorbancy had good linear relationship between 0.011 and 0.110 mg / mL. The regression equation was Y = 18.015X + 0.020; and the correlation coefficient was R2 = 0.999 4. The relative percentage of total flavonoids in H. Scandens was 1.609%. And the RSD in precision test was 1.10%. Conclusion This method was simple, reliable and could provide scientific references for the development and utilization of the medicinal value of H. scandens.