Background:Single-fiber electromyography (SFEMG) has been suggested as a quantitative method for supporting chronic partial denervation in amyotrophic lateral sclerosis (ALS) by the revised El Escorial criteria.Although concentric needle (CN) electrodes have been used to assess jitter in myasthenia gravis patients and healthy controls,there are few reports using CN electrodes to assess motor unit instability and denervation in neurogenic diseases.The aim of this study was to determine whether quantitative changes in jitter and spike number using CN electrodes could be used for ALS studies.Methods:Twenty-seven healthy controls and 23 ALS patients were studied using both CN and single-fiber needle (SFN) electrodes on the extensor digitorum communis muscle with an SFEMG program.The SFN-jitter and SFN-fiber density data were measured using SFN electrodes.The CN-jitter and spike number were measured using CN electrodes.Results:The mean CN-jitter was significantly increased in ALS patients (47.3 ± 17.0 μs) than in healthy controls (27.4 ± 3.3 μs) (P ＜ 0.001).Besides,the mean spike number was significantly increased in ALS patients (2.5 ± 0.5) than in healthy controls (1.7 ± 0.3) (P ＜ 0.001).The sensitivity and specificity in the diagnosis of ALS were 82.6％ and 92.6％ for CN-jitter (cut-off value:32 μs),and 91.3％ and 96.3％ for the spike number (cut-offvalue:2.0),respectively.There was no significant difference between the SFN-jitter and CN-jitter in ALS patients; meanwhile,there was no significant difference between the SFN-jitter and CN-jitter in healthy controls.Conclusion:CN-jitter and spike number could be used to quantitatively evaluate changes due to denervation-reinnervation in ALS.