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目的 探讨血管紧张素转换酶抑制剂 (ACEI)培哚普利干预慢性心衰对心肌肌浆网 (SR)Ca2 +泵活性和Ca2 +释放通道 (RyR2 )密度的影响及意义。方法 通过结扎大鼠左冠脉建立慢性心衰模型 ,以培哚普利进行干预 ,对照观察血流动力学、左室心肌SRCa2 +泵活性、[3H] ryanodine与RyR2 最大结合量 (Bmax)Kd值。结果 与对照组 (C组 )相比 ,心衰组 (F组 )LVEDP显著升高 (P <0 .0 1) ,+dp/dtmax、-dp/dtmax显著降低 (P <0 .0 1) ,培哚普利组 (P组 )LVEDP显著低于F组 (P <0 .0 1) ,+dp/dtmax、-dp/dtmax显著高于F组 (P <0 .0 1)。F组心肌SRCa2 +泵活性、[3H] ryanodine与RyR2 最大结合量Bmax显著低于C组 (P <0 .0 1) ,P组显著高于F组(P <0 .0 1) ,3组Kd值差异不显著 (P >0 .0 5 )。心肌SRCa2 +泵活性与 +dp/dtmax、-dp/dtmax显著正相关 (r =0 .5 16 1、r =0 .6 172 ,P <0 .0 1)。结论 培哚普利长期干预慢性心衰 ,能够改善心肌SRCa2 +泵活性 ,增加RyR2密度 ,可能与其改善心肌舒缩功能及心肌保护作用有关
Objective To investigate the effect and significance of perindopril with angiotensin converting enzyme inhibitor (ACEI) on the activity of Ca2 + pump and the density of Ca2 + release channel (RyR2) in myocardial sarcoplasmic reticulum (SR) after chronic heart failure. Methods Chronic heart failure model was established by ligation of the left coronary artery in rats. Perindopril intervention was performed. The hemodynamics, SRCa2 + pump activity, the maximum binding capacity of [3H] ryanodine to RyR2 (Kmax) value. Results Compared with the control group (C group), the LVEDP, FDP and dtmax were significantly lower in group F (P <0.01) LVEDP in perindopril group (P group) was significantly lower than that in F group (P <0.01), and dp / dtmax and -dp / dtmax in perindopril group were significantly higher than those in F group (P <0.01). The maximal binding capacity of [3H] ryanodine and RyR2 in group F was significantly lower than that in group C (P <0.01), P group was significantly higher than that in group F (P <0.01), group 3 Kd value difference was not significant (P> 0.05). Cardiac SRCa2 + pump activity was positively correlated with + dp / dtmax and -dp / dtmax (r = 0.5161, r = 0.626, P <0.01). Conclusion Perindopril can prolong myocardial SRCa2 + pump activity and increase RyR2 density in long-term intervention of chronic heart failure, which may be related to the improvement of myocardial relaxation and contractility and myocardial protection