论文部分内容阅读
目的:采用核素标记相对和绝对定量(iTRAQ)蛋白组学技术分析养心通脉有效部位方(apr-YTF)对骨髓间充质干细胞(BMSCs)的作用蛋白。方法:SD大鼠按照每天31.08 g·kg-1给予养心通脉有效部位方灌服,以灌服等量生理盐水大鼠含药血清为空白对照,5 d后取含药血清干预BMSCs,提取细胞的膜蛋白,运用液相色谱基质辅助激光解析/电离-飞行时间质谱技术(MALDI-TOF-MS)筛选并鉴定差异表达蛋白,并对其进行生物信息学分析。结果:鉴定出236个蛋白,差异蛋白62个,其中表达上调的蛋白有48个,表达下调的蛋白有14个;这些蛋白主要参与102种生物学过程,35种细胞组分,6种分子途径和3条信号转导通路,这些蛋白在3条信号转导通路上相互发生作用。结论:由结果推测处于差异蛋白功能交互网中交叉点位置的早老蛋白-1(presenilin-1),presenilin-2通过Notch信号通路,突触融合蛋白-4(syntaxin-4)通过可溶性N-乙基顺丁烯二酰亚胺敏感性的融合蛋白附着蛋白(SNARE)信号通路,丝裂原活化蛋白激酶12(MAPK12)通过MAPK信号通路在apr-YTF诱导BMSCs向心肌分化过程中发挥重要作用。
OBJECTIVE: To analyze the effect of apr-YTF on bone marrow mesenchymal stem cells (BMSCs) by proteomic techniques with radionuclide relative and absolute quantification (iTRAQ). Methods: Sprague-Dawley rats were fed with the active site of Yangxintongmai at a dose of 31.08 g · kg-1 every day. The rats were injected with equal volume of saline-containing serum as blank control. After 5 days, Cell membrane proteins were extracted and the differentially expressed proteins were screened by MALDI-TOF-MS and bioinformatics analysis was performed. RESULTS: Totally 236 proteins and 62 differential proteins were identified. Among them, 48 proteins were up-regulated and 14 proteins were down-regulated. These proteins were mainly involved in 102 biological processes, 35 cell components and 6 molecular pathways And three signal transduction pathways that interact with each other on three signal transduction pathways. CONCLUSIONS: Presenilin-1, presenilin-2, located at the crosspoint of the differential protein functional interaction network, are presumed to be activated by the N-acetylglucosamine signaling pathway through the Notch signaling pathway and syntaxin-4 (MAPK12) plays an important role in apr-YTF-induced BMSCs differentiation into cardiomyocytes via the MAPK signaling pathway, which is a sensitive fusion protein-attached protein (SNARE) signaling pathway based on maleimide.