1960年Sharwia用甲苯胺蓝活体染色来观察口腔内及咽喉的可疑病变,鉴别其良性或恶性,后者可着色。1963年Rithart用来染子宫颈粘膜的病损,对原位癌和上皮异常增殖区可染出其范围。1964年Niebel等强调这种简单测试方法可用在口腔粘膜病损,早 In 1960 Sharwia stained with toluidine blue in vivo to detect suspicious lesions in the mouth and throat and to identify benign or malignant lesions, which can be colored. In 1963 Rithart used to stain the lesions of the cervical mucous membranes, which can stain the area of ​​in situ carcinoma and abnormal epithelial proliferation. In 1964 Niebel et al emphasized that this simple test method can be used in oral mucosal lesions, as early as