论文部分内容阅读
目的了解广东地区人群抗-Mur发生频率、性质和来源以及Mur抗原的分布;建立献血者Mur稀有血型库服务于临床用血。方法以微量板的盐水法和聚凝胺法对广东省中山、深圳、江门、东莞、惠州市34 419名献血者做抗-Mur筛查,用抗-Mur做红细胞Mur抗原筛查,部分标本采用固相免疫吸附试验(SPIA)筛查。结果本组筛查对象共检出抗-Mur 48例,占0.14%(48/34 419),其中IgM占79.2%(38/48),IgM+IgG占16.7%(8/48),IgG占4.2%(2/48);献血者抗-Mur频率比较,无显著性差异(P>0.05);中山患者抗-Mur频率为0.17%(5/3010),与献血者比较无明显差异(P>0.05);中山地区,Mur抗原在献血者、患者中的发生率分别为7.31%(128/1 752)vs 7.14%(80/1 120),P>0.05,献血者男性和女性中分别为7.7%(72/931)vs 6.5%(33/504),P>0.05;本组Mur阳性者均为汉族,其中广东省籍贯占66.7%(70/105);建立了中山地区献血者Mur稀有血型库;SPIA技术从2 541份血浆标本中检出4例抗-Mur。结论 SPIA可以检出37℃活性有较高临床意义的抗-Mur,广东地区献血人群Mur血型抗体、抗原发生频率均明显高于Rh D血型抗体、抗原发生频率,不规则抗体检测应增加Mur阳性筛选红细胞,供受血者均应检测Mur血型是否相合,以进一步提高输血免疫安全性。
Objective To understand the frequency, nature and origin of anti-Mur and the distribution of Mur antigen in Guangdong population. To establish Mur rare blood bank of blood donors to serve clinical blood. Methods 34 419 blood donors from Zhongshan, Shenzhen, Jiangmen, Dongguan and Huizhou of Guangdong Province were screened by ELISA and polyacrylamide method. The samples were screened by anti-Mur for Mur antigen. Some samples Screening with solid phase immunosorbent assay (SPIA). Results A total of 48 anti-Mur antibodies (48/34 419) were detected in this panel, of which IgM accounted for 79.2% (38/48), IgM + IgG accounted for 16.7% (8/48), IgG accounted for 4.2% (2/48). There was no significant difference in anti-Mur frequency between donors and blood donors (P> 0.05). The anti-Maur frequency of Zhongshan patients was 0.17% (5/3010) > 0.05). The prevalence of Mur antigen in blood donors and patients in Zhongshan was 7.31% (128/1 752) vs 7.14% (80/1 120), respectively, P> 0.05. In male and female donors, 7.7% (72/931) vs 6.5% (33/504), P> 0.05; all of the Mur positive people in this group were Han nationality, of which 66.7% (70/105) were from Guangdong province; Murray Blood bank; SPIA technology from 2 541 plasma samples detected in 4 cases of anti-Mur. Conclusions SPIA can detect Mur anti-Mur antibody with high clinical significance at 37 ℃ and blood donors in Guangdong Province. The frequency of antigens was significantly higher than that of Rh blood group and antigens, and the detection of irregular antibodies should increase Mur positive Red blood cells for screening, blood recipients should be detected whether the blood type of Mur coincidence, in order to further improve blood transfusion immune safety.