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结合活体荧光成像技术,为基于β-环糊精的两亲性嵌段4臂聚合物(β-CD-[P(AA-co-MMA)-b-PVP]4)建立一种荧光标记方法。通过酯交换反应为罗丹明B分子中引入含有双键的甲基丙烯酸羟乙酯,然后通过双键聚合反应将罗丹明B标记到两亲性嵌段共聚物上。借助红外、荧光光谱对标记产物进行表征,并通过紫外分光光度法计算荧光标记产率;制备长春西汀(VP)荧光载药胶束,进行动物组织分布实验和小动物活体荧光成像实验,对比两个实验结果来验证荧光标记结果。荧光标记产率为4.13%;荧光标记前后该聚合物的临界胶束浓度(CMC)基本未改变,分别为5.09×10~(-3)和4.96×10~(-3) mg·L~(-1);对比组织分布和小动物活体荧光成像实验结果发现,二者反映的该胶束在小动物体内的分布情况基本相似,不仅证明了该标记产物的荧光示踪作用,同时结合红外、荧光表征也确证了此荧光标记实验的成功。结合活体荧光成像技术为两亲性嵌段共聚物建立一种荧光标记方法。
A fluorescent labeling method was developed for the amphiphilic block 4-arm polymer based on β-cyclodextrin (β-CD- [P (AA-co-MMA) -b-PVP] 4 with fluorescence imaging . Through the transesterification reaction, hydroxyethyl methacrylate containing double bonds is introduced into Rhodamine B molecule, and rhodamine B is then labeled to the amphiphilic block copolymer through double-bond polymerization. The labeled product was characterized by infrared and fluorescence spectroscopy, and the yield of fluorescent label was calculated by ultraviolet spectrophotometry. The vinpocetine (VP) fluorescent drug-loaded micelles were prepared for animal tissue distribution and live animal fluorescence imaging. Two experimental results to verify the fluorescent labeling results. The fluorescence labeling yield was 4.13%. The critical micelle concentration (CMC) of the polymer before and after fluorescence labeling remained unchanged at 5.09 × 10 -3 and 4.96 × 10 -3 mg · L -1 -1). The results of tissue distribution and fluorescence imaging of live animals showed that the distribution of the micelles in the small animals was basically similar, which not only proved the fluorescence tracing of the labeled products, but also combined the infrared, Fluorescence characterization also confirmed the success of this fluorescent labeling experiment. A fluorescent labeling method for amphiphilic block copolymer was established by living fluorescence imaging.