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目的:观察角膜板层膨体聚四氟乙烯植入物的孔径与角膜成纤维细胞长入程度的关系;研究其对角膜营养代谢的影响。方法:将孔径分别为45μm、28μm的膨体聚四氟乙烯(expanded polytetrafluoroethylene,ePTFE)制成8mm直径圆盘植入兔角膜板层中,裂隙灯观察并照相、光镜、透射电镜检查。结果:角膜成纤维细胞能长入两种孔径的,ePTFE微孔内并合成胶原,但小孔径植入物网孔内成纤维细胞数与胶原含量少于大孔径组;早期小孔径组出现植入物前板层角膜细胞数减少;大、小孔径两组分别有3眼与5眼(P>0.05)发生角膜脂肪变性;最长观察期7个月两组植入物稳定。结论:角膜成纤维细胞能长入孔径为28μm的ePTFE网孔中,但长入的速度与程度低于孔径为45μm的ePTFE;小孔径材料在早期影响角膜的营养代谢;无论孔径大小,ePTFE植入可导致一定程度的角膜脂肪变性,但不影响植入物的长期存留。孔径45μm的ePTFE是良好的人工角膜周边部材料。眼科学报1999;15:246-249。
OBJECTIVE: To observe the relationship between the aperture of expanded corneal polytetrafluoroethylene (PTFE) implants and the length of corneal fibroblasts, and to study their effects on corneal nutrition metabolism. Methods: 8mm diameter discs were implanted into rabbit corneal lamellae with 45μm and 28μm expanded polytetrafluoroethylene (ePTFE). The slit lamp was observed and photographed, light microscopy and transmission electron microscopy. Results: The corneal fibroblasts could grow into two kinds of ePTFE micropores and synthesize collagen. However, the number of fibroblasts and collagen in mesoporous mesoporous scaffolds were smaller than those in large aperture groups. The number of corneal cells in the lamina propria was reduced. Corneal steatosis occurred in 3 eyes and 5 eyes (P> 0.05) in the large and small aperture groups, respectively. The longest observation period was 7 months. CONCLUSION: Corneal fibroblasts can grow into ePTFE mesh with a pore size of 28μm, but the growth rate and degree of entrapment are lower than that of ePTFE with a pore size of 45μm. Small pore size material can affect the corneal nutrition metabolism at an early stage. Regardless of pore size, ePTFE Intrusions can cause some degree of corneal steatosis but do not affect the long-term survival of the implant. EPTFE with a pore size of 45 μm is a good artificial corneal peripheral material. Journal of Ophthalmology 1999; 15: 246-249.