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目的:探讨microRNA-106a(miR-106a)在上皮性卵巢癌(EOC)细胞迁移和侵袭中的作用,并验证TGFBR2是否系miR-106a的直接靶点。方法:选取具有不同转移能力的EOC细胞系(SKOV-3,A2780),用Real-time RT-PCR检测miR-106a在其中的表达。通过transwell实验,体外分析miR-106a是否具有促进EOC细胞系迁移和侵袭的作用。通过稳定性表达转化生长因子-β受体2(TGFBR2)或者沉默TGFBR2,探讨了miR-106a可能的作用靶点。结果:miR-106a在转移性EOC细胞(SKOV-3)中高表达(P<0.05),可在体外促进EOC细胞的迁移和侵袭(P<0.05)。这些表型上的改变并非由于miR-106a可同时促进癌症细胞的增殖所引起。miR-106a促进EOC细胞迁移和侵袭的作用是通过抑制TGFBR2来实现的。结论:miR-106a可通过直接抑制抗转移分子而促进EOC细胞的迁移和侵袭。
Objective: To investigate the role of microRNA-106a (miR-106a) in the migration and invasion of epithelial ovarian cancer (EOC) cells and to verify whether TGFBR2 is the direct target of miR-106a. Methods: EOC cell lines with different metastatic potential (SKOV-3, A2780) were selected and the expression of miR-106a was detected by Real-time RT-PCR. Through transwell experiments, in vitro analysis of whether miR-106a promotes the migration and invasion of EOC cell lines. The possible targets of miR-106a were explored by stably expressing transforming growth factor-β receptor 2 (TGFBR2) or silencing TGFBR2. Results: miR-106a was highly expressed in the metastatic EOC cells (SKOV-3) (P <0.05), which could promote the migration and invasion of EOC cells in vitro (P <0.05). These phenotypic changes are not caused by the simultaneous promotion of proliferation of cancer cells by miR-106a. The role of miR-106a in promoting EOC cell migration and invasion is achieved by inhibiting TGFBR2. Conclusion: miR-106a can promote the migration and invasion of EOC cells by directly inhibiting anti-metastasis molecules.