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试验表明,以黄瓜幼苗的生长点为外植体时,一定浓度的NAA和KT配比使用为黄瓜试管成花所必需。在MS培养基中附加0.1毫升/升或0.5毫升/升NAA的情况下,KT在1.0～2.5毫克/升范围内与花芽形成率和开花率成正相关,与愈伤化成负相关;而在添加1.0～2.5毫克/升KT的情况下,0.5毫克/升的NAA较0.1毫克/升NAA有更强的愈伤化和促进茎生长的作用。在添加0.5毫克/升和2.5毫克/升KT的情况下,黄瓜花芽形成率和开花率最高,分别达44.4%和33.3%。
Experiments show that the growth point of cucumber seedlings as explants, a certain concentration of NAA and KT ratio required for the cucumber tube into flowers. KT was positively correlated with flower bud formation rate and flowering rate in the range of 1.0-2.5 mg / L, and negatively correlated with callus when MS medium was supplemented with 0.1 mL / L or 0.5 mL / L NAA. However, At 1.0-2.5 mg / L KT, 0.5 mg / L NAA had stronger callus and stem growth promotion effects than 0.1 mg / L NAA. With 0.5 mg / L and 2.5 mg / L KT, cucumber flower bud formation rate and flowering rate were the highest, reaching 44.4% and 33.3% respectively.