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目的观察蒿甲醚体外抗多房棘球绦虫原头蚴作用,探讨治疗泡球蚴病新药物。方法将多房棘球绦虫原头蚴随机分为4组:蒿甲醚低剂量组(50μg/ml),蒿甲醚高剂量组(100μg/ml),阿苯达唑组(50μg/ml),空白对照组。每组设2个平行组,每组约含3 000个原头节,分别置含10 ml DMEM培养液的培养瓶中,每天观察1次,取样滴于玻片,用0.03%美蓝染色,显微镜下计数原头蚴约200个,计算死亡率。直至该组原头蚴全部死亡时培养结束,并对原头蚴进行超微结构观察。结果蒿甲醚50μg/ml和100μg/ml组不同作用时间原头蚴死亡率与空白对照组相比差异均有统计学意义(P<0.05);高、低剂量组原头蚴死亡率差异无统计学意义(P>0.05)。培养至第9 d时,蒿甲醚高剂量组原头蚴死亡率达100%,蒿甲醚低剂量组原头蚴死亡率93.28%,阿苯达唑组原头蚴死亡率99.03%,空白对照组原头蚴死亡率78.83%。结论蒿甲醚具有体外抗多房棘球绦虫原头蚴作用,且呈剂量、时间依赖。
Objective To observe the effect of artemether on the protoscoiidae of Echinococcus multilocularis in vitro and explore the new drug for the treatment of alveolar hydatid disease. Methods Echinococcus multilocularis was divided into 4 groups at random: low dose artemether group (50μg / ml), high dose artemether group (100μg / ml), albendazole group (50μg / ml) ,Blank control group. Each group with 2 parallel groups, each containing about 3 000 of the original head section, respectively, containing 10 ml of DMEM culture flask, observed once a day, the sample was dropped on glass slides, stained with 0.03% methylene blue, Under the microscope, there were about 200 protoscoleces and calculated the mortality rate. Until the group of all the original cercariae died when the culture ended, and the original cercariae ultrastructural observation. Results There were significant differences in the mortality of the protoscoleces (P <0.05) between the groups treated with artemether at 50μg / ml and 100μg / ml for different time periods. Statistical significance (P> 0.05). In the 9th day, the mortality of the protoscoleces was 100% in the high-dose artemether group, 93.28% in the low-dose artemether group, 99.03% in the albendazole-treated group and 99.03% in the albendazole group The control group, the original mortality of 78.83%. Conclusion Artemether has anti-Echinococcus multilocularis activity in vitro and in dose and time-dependent manner.