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目的采取人工外淋巴灌流方法建立动物模型，是耳蜗生物学特性研究的新方法，但其成功率较低。为提高人工外淋巴灌流实验的成功率，本实验探索和改进了灌流管的封固和灌流速度等实验技术。方法通过安放圆窗电极监测由短声诱发的听神经复合动作电位（compoundactionpotentialCAP）阈值、扫描电镜观察动物颞骨标本，在生理功能和形态学上予以验证此方法的可靠性。结果灌流前CAP阈值41．00±3．16dBSPL，灌流2小时（或少数动物2．5小时）后为42．00±2．18dBSPL，CAP阈值灌流前、后未见明显变化。扫电镜观察内、外毛细胞的形态结构与对照耳相比未见明显变化。结论生理指标及形态学观察结果均证明，改进后的灌流方法可行。
OBJECTIVE: To establish a animal model of artificial peripheral lymph perfusion is a new method to study the biological characteristics of cochlear, but its success rate is low. In order to improve the success rate of artificial external lymphatic perfusion experiment, this experiment explored and improved experimental techniques such as the sealing and perfusion rate of perfusion tube. Methods The threshold of auditory compound action potential (CAP) evoked by short beacons was monitored by round-window electrodes and the temporal bone samples were observed by scanning electron microscopy to verify the reliability of this method in physiological function and morphology. Results Before perfusion, the threshold value of CAP was 41.00 ± 3.16 dBSPL, 42.00 ± 2.18 dB SPL after perfusion for 2 hours (or a few animals for 2.5 hours). There was no significant change before and after CAP threshold perfusion. Scanning electron microscopy observation showed that there was no significant change in the morphology of the outer hair cells compared with the control ears. Conclusion Physiological indexes and morphological observation results show that the improved perfusion method is feasible.