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目的探讨甲醛诱导DNA-蛋白质交联作用及其修复效应。方法采用培养V79细胞株作为实验材料,以不同浓度的液态甲醛(0、50、100、200、400、800、1 600μmol/L)对细胞染毒1 h后检测DPC率,并根据实验结果选取200μmol/L对培养细胞株进行修复实验(修复0、6、12、18、24 h)。采用KCl-SDS沉淀法检测DPC率。结果较高浓度的液态甲醛(≥200μmol/L)可以产生明显的DPC效应(P<0.05);由200μmol/L浓度的液态甲醛诱导V79细胞产生明显的DPC(P<0.05),经过修复可以恢复到空白对照水平(P>0.05)。结论较高浓度的甲醛可以明显诱导DPC形成,甲醛所致的DNA-蛋白质交联可以得到修复。
Objective To investigate the formaldehyde-induced DNA-protein cross-linking and its repair effect. Methods The V79 cell line was used as the experimental material to detect the DPC rate after being exposed to different concentrations of liquid formaldehyde (0, 50, 100, 200, 400, 800 and 600 μmol / L) for 1 h and selected according to the experimental results 200μmol / L cultured cell line repair experiment (repair 0,6,12,18,24 h). DPC rate was determined by KCl-SDS precipitation. RESULTS: DPC was significantly induced by higher concentrations of liquid formaldehyde (≥200μmol / L) (P <0.05). DPC was significantly induced by liquid formaldehyde at a concentration of 200μmol / L (P <0.05) To the blank control level (P> 0.05). Conclusions Higher concentration of formaldehyde can obviously induce the formation of DPC, and formaldehyde-induced DNA-protein cross-linking can be repaired.