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采用~(51)Cr 释放法以鸡红细胞作靶细胞,分离单个核细胞作效应细胞进行 ADCC 测定,并对主要试验影响因素进行了探讨。对37例正常人,81例乙型肝炎病人及15例带抗原者进行 ADCC 测定和随访观察。各型肝炎病人ADCC 皆有不同程度减低,但以重症肝炎及慢性活动性肝炎病人明显,ADCC 减低和肝炎型别、病期、病情及生化改变密切相关,因此 ADCC 测定具有临床应用价值。对19例乙型肝炎病人进行了 ADCC 血清抑制活性测定。通过去抑制或再生试验,证明了 ADCC 减低系由于血清抑制作用。发现乙型肝炎病人 E-玫瑰花形成抑制因子(RIF)阳性血清有抑制 ADCC 活性的效应。
Using the method of ~ (51) Cr release, chicken erythrocytes were used as target cells, and mononuclear cells were isolated as effector cells for ADCC determination. The influencing factors of the main test were also discussed. ADCC was measured and followed up in 37 normal subjects, 81 hepatitis B patients and 15 patients with antigens. ADCC in patients with various types of hepatitis are reduced to varying degrees, but in patients with severe hepatitis and chronic active hepatitis obvious, ADCC reduction and hepatitis type, duration, disease and biochemical changes are closely related, so the determination of ADCC has clinical value. ADCC serum anti-tumor activity was measured in 19 hepatitis B patients. Depletion of ADCC was demonstrated by serum inhibition by de-inhibition or regeneration experiments. Hepatitis B patients found that E-rose inhibitory factor (RIF) -positive serum has the effect of inhibiting ADCC activity.