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目的建立HPLC-PDA法在最大吸收波长处分别测定芪芍方有效部位中主要有效成分葛根素、大豆苷、芍药苷含量的方法。方法采用ODSHYPERSIL色谱柱(250 mm×4.6 mm,5μm),乙腈-0.2%磷酸水梯度洗脱,柱温:35℃,流速0.8 mL/min,采用250 nm、230 nm 2个检测波长对葛根素、大豆苷和芍药苷进行含量测定。结果葛根素、大豆苷和芍药苷分别在0.096 4～1.542 4μg、0.094 4～1.510 4μg、0.099 6～1.593 6μg的范围内线性关系良好,平均加样回收率分别为97.44%、98.40%、100.26%,RSD分别为1.31%、2.70%、1.35%。结论该方法简便、准确、重复性好,可以用于芪芍方有效部位的质量控制。
OBJECTIVE To establish a HPLC-PDA method for determination of puerarin, daidzin and paeoniflorin in the active fractions of Qi Shao Fang at the maximum absorption wavelength. Methods ODSHYPERSIL column (250 mm × 4.6 mm, 5 μm) was used to elute gradient of acetonitrile-0.2% phosphoric acid. The column temperature was 35 ℃ and the flow rate was 0.8 mL / min. The detection wavelength was 250 nm and 230 nm. , Daidzin and paeoniflorin were determined. Results Puerarin, daidzin and paeoniflorin showed good linearity in the range of 0.096 4 ~ 1.542 4μg, 0.094 4 ~ 1.510 4μg and 0.099 6 ~ 1.593 6μg, respectively. The average recoveries were 97.44%, 98.40% and 100.26%, respectively. , RSD respectively 1.31%, 2.70%, 1.35%. Conclusion The method is simple, accurate and reproducible. It can be used for the quality control of the active fractions of Qi Shao Fang.