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目的 研究糖尿病大鼠下丘脑和胰腺神经肽Y的mRNA及蛋白表达情况 ,以探讨NPY和糖尿病的关系。方法 建立链脲佐菌素 (STZ)诱导的糖尿病大鼠模型 ,设立糖尿病、糖尿病胰岛素治疗及对照组 ,饲养 2 4周后处死动物。用原位杂交和免疫组化检测下丘脑和胰腺NPY的mRNA及蛋白表达情况。结果 1.糖尿病大鼠下丘脑NPY的mRNA及蛋白表达均增加 ,NPY的mRNA表达主要在弓状核 ,说明弓状核是NPY产生的主要部位。 2 .给予胰岛素治疗后 ,STZ糖尿病大鼠下丘脑的NPY的mRNA及蛋白表达均较糖尿病组明显下降 ,说明胰岛素缺乏是STZ糖尿病大鼠下丘脑NPY增多的重要原因之一。 3.下丘脑NPY增高的糖尿病大鼠具有多饮、多食 ;胰岛素治疗后其NPY下降 ,糖尿病大鼠的多饮、多食明显缓解 ,证实NPY参与STZ糖尿病大鼠的某些病理生理过程。 4 .首次揭示在STZ糖尿病大鼠胰岛中NPY的mRNA及蛋白表达增加。胰岛素NPY阳性细胞的分布也发生改变 ,由主要分布在周边变为整个胰岛散在分布 ,给予胰岛素治疗后 ,胰岛中的NPY和mRNA及蛋白表达未见明显改变。结论 糖尿病下丘脑NPY的增多 ,主要参与糖尿病的多饮、多食等病理生理过程。虽然糖尿病胰岛的NPY增多原因及其作用还不完全清楚 ,但为今后进一步的研究提供了重要线索。
Objective To study the mRNA and protein expression of neuropeptide Y in the hypothalamus and pancreas in diabetic rats to explore the relationship between NPY and diabetes mellitus. Methods Streptozotocin (STZ) -induced diabetic rat model was established. Diabetes mellitus and diabetic insulin therapy and control group were established. Animals were sacrificed after 24 weeks. The mRNA and protein expression of NPY in hypothalamus and pancreas were detected by in situ hybridization and immunohistochemistry. NPY mRNA and protein expression in the hypothalamus of diabetic rats were increased, NPY mRNA expression mainly in the arcuate nucleus, indicating arcuate nucleus is the main part of NPY production. After insulin treatment, NPY mRNA and protein expression in the hypothalamus of STZ diabetic rats were significantly lower than those in diabetic patients, indicating that insulin deficiency is one of the important causes of hypothalamus NPY increase in STZ diabetic rats. NPY increased hypothalamus in diabetic rats with more drink, eat more; insulin treatment decreased NPY, diabetic rats to drink more, eat significantly relieved, NPY involved in certain pathophysiological processes STZ diabetic rats. It was the first time to reveal the increase of NPY mRNA and protein in islets of STZ diabetic rats. The distribution of insulin NPY positive cells also changed, from the main distribution in the periphery scattered into the entire islet, given insulin treatment, islet NPY and mRNA and protein expression no significant change. Conclusions The increase of hypothalamus NPY in diabetes mellitus is mainly involved in the pathophysiological process of diabetes, such as polydipsia and polyphagia. Although the causes of NPY increase in diabetic islets and their roles are not fully understood, they provide important clues for further research in the future.