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研究长期使用肾上腺素能受体阻断剂治疗对慢性压力超负荷左心室电重构的影响。新西兰兔通过肾上腹主动脉次全结扎诱发慢性压力超负荷,10周后行心脏超声检查,并采用全细胞膜片钳技术分别记录腹主动脉结扎组(简称结扎组)、腹主动脉结扎+Carvedilol 干预组(简称Carvedilol组)及正常对照组(简称对照组)动物左室肌中层细胞的动作电位(action potential,AP)、内向整流钾电流(inward rectifier potassium current,IKi)、延迟整流钾电流(delayed rectifier potassium current,IK)及Na+/Ca2+交换体电流。结果表明,结扎组的左室质量指数较对照组明显升高,Carvedilol组较结扎组明显降低(P<0.01)。在2 s的基础周长下,动作电位持续时间(以90％的复极时间表示,简称APD90)在对照组、结扎组及Carvedilol组分别为522.0±19.5 ms(n=6)、664.7± 46.2 ms(n=7)、567.8±14.3 ms(n=8),结扎组同对照组相比,P<0.01,Carvedilol组同结扎组相比,P<0.05。在测试电位为-100mV时,IKi电流密度(pA/pF)在对照组、结扎组及Carvedilol组分别为-11.8±0.50(n=8),-8.07±0.28 (n=8),-10.69±0.35(n=8),结扎组与对照组及Carvedilol组相比,P<0.01。在测试电位为+50 mV时,IK尾电流密度(pA/pF)在对照组、结扎组及Carvedilol组分别为0.59±0.40(n=
To study the effects of long-term adrenergic receptor blocker therapy on left ventricular remodeling in chronic stress overload. New Zealand rabbits underwent chronic pressure overload by sub-total abdominal aorta ligation. Cardiac echocardiography was performed 10 weeks later. The whole-cell patch-clamp technique was used to record the abdominal aorta ligation group (abbreviated as ligation group), abdominal aorta ligation + Carvedilol intervention (AP), inward rectifier potassium current (IKi), delayed rectifier potassium current (delayed rectifier potassium current) in the left ventricular muscle of rats in the control group (group Carvedilol) and normal control group rectifier potassium current, IK) and Na + / Ca2 + exchanger current. The results showed that left ventricular mass index of ligation group was significantly higher than that of control group, and Carvedilol group was significantly lower than that of ligation group (P <0.01). The duration of action potential (referred to as APD90 for 90% of the repolarization time) was 522.0 ± 19.5 ms (n = 6) and 664.7 ± 46.2 (n = 6) in the control group, ligation group and the Carvedilol group, respectively. (n = 7), 567.8 ± 14.3 ms (n = 8), P <0.01 in the ligation group compared with the control group, and P <0.05 in the Carvedilol group compared with the ligation group. Iki current density (pA / pF) was -11.8 ± 0.50 (n = 8), -8.07 ± 0.28 (n = 8), -10.69 ± (n = 8) in the control group, ligation group and the Carvedilol group at the test potential of -100 mV 0.35 (n = 8), P <0.01 in ligation group compared with control group and Carvedilol group. At a test potential of +50 mV, IK tail current density (pA / pF) was 0.59 ± 0.40 in the control, ligation and Carvedilol groups (n =