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目的 研究丙型肝炎 (简称丙肝 )病毒基因分型和病毒负荷与干扰素抗病毒治疗的应答关系。方法 用特异性引物PCR的改良法检测基因型 ,用荧光PCR方法检测病毒负荷 ,接受干扰素治疗的慢性丙肝患者根据不同的基因型及病毒负荷进行应答分组比较。结果 治疗前HCV -RNA负荷小于 10 9eq·L-1患者经干扰素治疗后表现为持续应答状态占总人数的 4 2 9% ,HCV -RNA负荷大于 10 9eq·L-1患者经干扰素治疗后呈部分应答(2 8 6 % )或无应答状态 (2 8 6 % )。统计学上差异非常显著 (P <0 0 0 1)。 2a型和 1b +2a型 (共 11例 )疗效明显优于 1b型 (2 2例 ) ,差异非常显著 (P <0 0 0 1)。结论 对HCV -RNA负荷小于 10 9eq·L-1及 2a +1b、2a型患者干扰素治疗效果较好。HCV病毒负荷及基因分型可能影响对干扰素治疗的应答。
Objective To study the genotypes of hepatitis C virus (HCV) and the relationship between viral load and anti-viral treatment of interferon. Methods Genotypes were detected by PCR with specific primers. The viral load was detected by fluorescent PCR. The patients with chronic hepatitis C treated with interferon were grouped according to different genotypes and viral loads. Results The patients with HCV-RNA load of less than 109 eq · L-1 before treatment had a sustained response status of 42.9% after interferon treatment and those with HCV-RNA load greater than 109 eq · L-1 were treated with interferon After partial response (286%) or no response (286%). The difference was statistically significant (P <0.01). The efficacy of type 2a and 1b + 2a (11 patients in total) was significantly better than that of type 1b (22 patients), with significant difference (P <0.01). Conclusions Interferon treatment is better in type 2a patients with HCV-RNA load less than 109eq · L-1 and 2a + 1b. HCV viral load and genotyping may affect response to interferon treatment.