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目的探讨红芪总黄酮对高强度离心运动大鼠耐力及骨骼肌中一氧化氮(NO)和一氧化氮合酶(NOS)的影响。方法将72只Wistar大鼠随机分为安静对照组、离心运动组和药物预处理组,各组再依据运动前后时间点不同分为运动前1h,运动后1,24,48h4个亚组,每组6只。离心运动前3d,药物预处理组大鼠每日定时给予0.2mg/kg红芪总黄酮灌胃,其余各组大鼠给予等量生理盐水灌胃,观察大鼠最长离心运动时间。离心运动后,取各组大鼠腓肠肌,制备匀浆液,检测NO含量及NOS活性。结果药物预处理组运动时间为(86.25±7.97)min,与离心运动组的(73.12±5.23)min比较差异有统计意义(P<0.05)。离心运动后1h,离心运动组大鼠骨骼肌中NO含量急剧下降(P<0.01),运动后24h,NO含量开始回升,至运动后48h,NO含量接近正常水平;红芪总黄酮可减缓离心运动后大鼠骨骼肌中NO含量的下降(P<0.05),对NOS的表达及活性没有影响。结论红芪总黄酮可增强高强度离心运动大鼠耐力,其机制可能与其减缓骨骼肌中NO含量的急剧下降有关。
Objective To investigate the effects of total flavone of Radix Hedysari on endurance and the levels of nitric oxide (NO) and nitric oxide synthase (NOS) in skeletal muscle of high-intensity centrifuge rats. Methods Seventy two Wistar rats were randomly divided into control group, eccentric exercise group and drug pretreatment group. Each group was divided into 4 groups according to different time points before exercise: 1 hour before exercise, 1,24,48 hours after exercise, 4 subgroups Group of six. Three days before eccentric exercise, the rats in the drug pretreatment group were given 0.2mg / kg of total flavonoids of Radix Houttuyniae Officinalis every other day for gavage. The rats in other groups were administered with the same amount of physiological saline, and the longest time of eccentric exercise was observed. After centrifugation, the gastrocnemius muscle of each group was taken and the homogenate was prepared to detect the content of NO and the activity of NOS. Results The exercise time in the drug pretreatment group was (86.25 ± 7.97) min, which was significantly lower than that in the eccentric exercise group (73.12 ± 5.23) min (P <0.05). At 1h after eccentric exercise, the content of NO in skeletal muscle of the rats in the eccentric exercise group dropped sharply (P <0.01), and the content of NO began to rise after 24 hours of exercise. The content of NO was close to the normal level 48h after exercise. After exercise, the content of NO in skeletal muscle of rats decreased (P <0.05), and had no effect on the expression and activity of NOS. Conclusion The total flavone of Radix Hedysari can enhance the endurance of rats in high-intensity eccentric exercise, and its mechanism may be related to the rapid decrease of NO in skeletal muscle.