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本文观察了40只家鸽壶腹嵴于不同低温(-40℃至-160℃)、时间(6、9分钟)冷冻后的内耳功能和前庭感觉上皮改变。结果:早期为冷冻-70℃9分钟开始形态有轻度改变。-90℃6分钟改变明显,感觉上皮细胞浆内线粒体损害,大量空泡形成,纤毛融解。更深低温显示细胞分解的严重破坏。晚期改变程度近似早期,前庭功能发生相应变化。耳蜗功能和形态无损。研究得出,本术可选择性地破坏壶腹嵴上皮细胞降低其功能,选用低温、时间以-90℃6分钟为宜。
In this paper, the inner ear function and the vestibular sensory epithelium of 40 pigeons were observed after cryopreservation (-40 ℃ to -160 ℃) and time (6 and 9 minutes). Results: In the early stage, there was a slight change in the morphology at the beginning of freezing -70 ° C for 9 minutes. -90 ℃ 6 minutes to change significantly, feeling mitochondrial damage in epithelial cells plasma, a large number of vacuoles formation, cilia melt. Darker temperatures show severe disruption of cell breakdown. Early changes in the degree of early stage, corresponding changes in vestibular function. Cochlear function and morphology are non-destructive. Studies have concluded that this technique can selectively destroy the ampulla of crest epithelial cells to reduce its function, the selection of low temperature, time to -90 ℃ 6 minutes is appropriate.