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目的观察司来吉兰对帕金森病(Parkinson disease,PD)模型大鼠胃功能障碍及胃窦酪氨酸羟化酶(tyrosine hydroxylase,TH)及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响,探讨司来吉兰对PD胃功能障碍的治疗作用及可能机制。方法 72只健康SD大鼠随机分为正常对照组、PD模型组和司来吉兰治疗组,后两组采用颈背部皮下注射鱼藤酮制备PD模型,模型制备成功后,模型组每日并灌胃生理盐水,治疗组每日灌胃给药司来吉兰0.5mg/kg。分别于治疗后4d、8d测定胃固体食物残留率,并采用免疫组化法和蛋白质印迹法检测胃窦TH和nNOS的表达。结果与对照组相比,模型组各时间点胃内固体食物残留率均增加,胃窦TH表达均减少,nNOS表达均增加,差异有统计学意义(P<0.01)。与模型组相比,治疗组各时间点胃内固体食物残留率均降低,胃窦TH表达均升高,nNOS表达均降低,差异有统计学意义(P<0.05或P<0.01)。与治疗4d组相比,治疗8d组大鼠胃内固体食物残留率明显降低,TH表达明显升高,nNOS阳性细胞表达明显降低,差异均有统计学意义(P<0.05)。结论司来吉兰可改善PD大鼠胃功能障碍,其作用机制可能与其减轻PD模型大鼠胃窦多巴胺能神经元的损伤和抑制nNOS表达有关。
Objective To observe the effect of selegiline on gastric dysfunction and tyrosine hydroxylase (TH) and neuronal nitric oxide (NO) in gastric mucosa of Parkinson disease (PD) synthase, nNOS), and discuss the therapeutic effect and possible mechanism of selegiline on PD gastric dysfunction. Methods Seventy-two healthy SD rats were randomly divided into normal control group, PD model group and selegiline treatment group. The latter two groups were treated with subcutaneous injection of rotenone on the dorsal neck to prepare PD model. After the model was successfully prepared, Saline, the treatment group daily intragastric administration of selegiline 0.5mg / kg. Residual gastric contents were measured 4 days and 8 days after treatment, and the expressions of TH and nNOS in gastric antrum were detected by immunohistochemistry and Western blot. Results Compared with the control group, the residual gastric food was increased at each time point in the model group, while the expression of TH in gastric antrum was decreased and the expression of nNOS was increased. The difference was statistically significant (P <0.01). Compared with the model group, the residual food in the stomach at each time point decreased, the expression of TH in gastric antrum increased and the expression of nNOS decreased. The difference was statistically significant (P <0.05 or P <0.01). Compared with the 4-day treatment group, the residual food in the stomach of the rats in the 8-day treatment group was significantly lower, the TH expression was significantly higher, and the expression of nNOS-positive cells was significantly lower (all P <0.05). Conclusion Administration of selegiline can improve gastric dysfunction in rats with PD. Its mechanism may be related to its ability to reduce the damage of dopaminergic neurons and the inhibition of nNOS expression.