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人们知道某些细菌细胞及其产物具有抗肿瘤作用已经100多年了.非经肠道给予实验动物或病人细菌产物,诸如脂多糖(LPS),可导致肿瘤内的严重出血反应.然而给予有效剂量的LPS常可引起循环性虚脱,以至死亡.但以其它免疫刺激剂处理,则往往可以见到肿瘤的消退.1975年Carswell报道,在经内毒素处理的荷瘤小鼠血清中存在着一种使肿瘤坏死的因子,并称之为肿瘤坏死因子(tumor necrosis factor,TNF).它可引起体内肿瘤的出血性坏死,对体外培养的癌细胞也具有细胞毒作用.TNF被认为是由巨噬细胞产生的.可以从内毒素休克的预致敏(Presensitized)动物血清中提取.LPS则属于TNF的刺激剂.本文的目的在于研讨TNF产生的条件及其动力学.结果表明,在有脂多糖存在的条件下,某些活化剂(Prming agents)诸如BCG、短小棒状杆菌及酵母聚糖(Zymosan)等,可以刺激机体的网织内皮系统(RES),因而是极好的TNF产生的诱导物.但这些诱导物在刺激产生的TNF方面有较大的差异性,主要取决于对RES的刺激程度.某些可诱导RES增生的物质,如痤疮丙酸菌(Propionibacterium acnes)和厌氧杆菌(Corynebacteriumanaerobium)因可诱发明显的RES增生,因而也可诱发高水平的TNF活性.然而PPD、OK432、PSK以及Choreito并不能诱发TNF的活性.鉴于上述观察,作者认为凡能诱导高水平RES增生的活化剂,均可诱发高水平的TNF活性.由此推论,痤疮丙酸菌是最好的TNF诱导剂.
It has been known for over 100 years that certain bacterial cells and their products have anti-tumor effects.Parenteral administration of laboratory animals or patients with bacterial products such as lipopolysaccharide (LPS) can result in severe hemorrhagic reactions in tumors However, administration of an effective dose Of LPS can often cause cyclic collapse and even death.However, treatment with other immunostimulants often can see the tumor regression.Cavallwell reported in 1975, in endotoxin-treated mice with tumor-bearing mice there is a The factor that causes tumor necrosis is called tumor necrosis factor (TNF), which causes hemorrhagic necrosis of tumors in vivo and also has cytotoxic effect on cancer cells cultured in vitro.TNF is thought to be produced by macrophages Cells can be extracted from the serum of presensitized animals of endotoxic shock.LPS belongs to the stimulator of TNF.The purpose of this paper is to investigate the conditions of TNF production and its kinetics.The results show that in the presence of lipopolysaccharide In the presence of certain priming agents such as BCG, Corynebacterium parvum and Zymosan, it stimulates the body’s reticuloendothelial system (RES) and is therefore an excellent TNF However, these inducers have significant differences in stimulating TNF production, depending on the extent of stimulation of RES Some substances that induce RES proliferation, such as Propionibacterium acnes and However, PPD, OK432, PSK and Choreito did not induce TNF activity.In view of the above observations, the authors believe that all of them can induce high level of TNF activity, RES proliferative activator, can induce high levels of TNF activity.According to inference, propionibacterium acnes is the best TNF inducer.