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目的:探讨磷脂酰肌醇3-激酶(PI3K)、存活素(Survivin)及血管内皮生长因子(VEGF)蛋白在人直肠癌组织中的表达,及其与直肠癌临床分期、组织学分级和侵袭转移的相关性及意义。方法:免疫组化SP法检测PI3K、Survivin和VEGF蛋白在60例直肠癌、30例直肠腺瘤及10例正常直肠组织中的表达。结果:PI3K在直肠癌、腺瘤和正常组织中的异常表达率分别为81.7%(49/60)、36.7%(11/30)和30.0%(3/10),差异有统计学意义,χ2=17.86,P=0.001;Survivin的阳性表达率分别为90.0%(54/60)、80.0%(24/30)和20.0%(2/10),差异有统计学意义,χ2=9.54,P=0.002;VEGF的异常表达率分别为71.7%(43/60)、43.3%(13/30)和30.0%(3/10),差异有统计学意义,χ2=5.68,P=0.025。PI3K、Survivin及VEGF的异常表达均与临床分期及转移密切相关,P值均<0.05。VEGF在直肠癌组织中染色阳性者(11.04±1.68)微血管密度(MVD)较染色阴性者(7.66±1.22)高,t=7.53,P<0.05。在直肠癌组织中PI3K与Survivin(r=0.396,P=0.001)和VEGF(r=0.417,P=0.000 5)表达呈正相关,Survivin与VEGF表达呈正相关,r=0.332,P=0.005。PI3K和Survivin及VEGF蛋白协同阳性表达的侵袭转移率为55.0%(33/60),其中Ⅳ期远处转移率为93.8%(15/16)。结论:PI3K和Survivin及VEGF蛋白的表达与临床分期及肿瘤侵袭转移密切相关,提示PI3K和Survivin及VEGF信号转导通路在人直肠癌侵袭转移中起重要作用。
Objective: To investigate the expression of phosphatidylinositol 3-kinase (PI3K), survivin and vascular endothelial growth factor (VEGF) in human rectal cancer and its relationship with clinical stage, histological grade and invasion Relevance and meaning of transfer. Methods: The expressions of PI3K, Survivin and VEGF protein in 60 cases of rectal cancer, 30 cases of rectal adenomas and 10 cases of normal rectal tissues were detected by immunohistochemical SP method. Results: The abnormal expression rates of PI3K in rectal cancer, adenoma and normal tissues were 81.7% (49/60), 36.7% (11/30) and 30.0% (3/10), respectively, the difference was statistically significant (χ2 = 17.86, P = 0.001 respectively). The positive rates of Survivin expression were 90.0% (54/60), 80.0% (24/30) and 20.0% (2/10) respectively. The difference was statistically significant 0.002 respectively. The abnormal expression rates of VEGF were 71.7% (43/60), 43.3% (13/30) and 30.0% (3/10), respectively. The difference was statistically significant (χ2 = 5.68, P = 0.025). The abnormal expression of PI3K, Survivin and VEGF were closely related to clinical stage and metastasis, P <0.05. The positive staining of VEGF in colorectal cancer (11.04 ± 1.68) was higher than that of negative staining (7.66 ± 1.22), t = 7.53, P <0.05. There was a positive correlation between PI3K expression and Survivin (r = 0.396, P = 0.001) and VEGF (r = 0.417, P = 0.0005) in rectal cancer tissues, and there was a positive correlation between Survivin and VEGF expression (r = 0.332, P = 0.005). The invasion and metastasis rates of PI3K, Survivin and VEGF protein were 55.0% (33/60), of which 93.8% (15/16) distant metastasis in stage Ⅳ. CONCLUSION: The expressions of PI3K, Survivin and VEGF are closely related to clinical stage and tumor invasion and metastasis, suggesting that PI3K, Survivin and VEGF signaling pathways play an important role in the invasion and metastasis of human rectal cancer.