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目的:初步探索NF-κB和HIF-1在宫颈上皮内瘤变和宫颈鳞癌中的表达及临床病理学意义。方法:以宫颈上皮内瘤变和宫颈鳞癌病例为研究对象,应用免疫组织化学方法检测NF-κB和HIF-1的表达情况并分析其表达在宫颈癌变中的意义。结果:NF-κB在正常宫颈上皮、CINⅠ、CINⅡ、CINⅢ、鳞癌的阳性表达率分别为16%,36%,44%,68%,72%。总体比较存在显著性差异x2=21.636,p<0.01。组间两两比较显示正常上皮与CINⅢ和鳞癌有显著性差异。HIF-1α在正常宫颈上皮、CINⅠ、CINⅡ、CINⅢ、鳞癌的阳性表达率分别为12%,20%,40%,68%,76%。总体比较存在显著性差异x2=32.733,p<0.01。组间两两比较显示正常上皮与CINⅢ(x2=16.333 p<0.001)和鳞癌(x2=20.779 p<0.001)有显著性差异。结论:NF-κB和HIF-1与宫颈鳞癌的发生有关,可能作为早期诊断的标志物。
OBJECTIVE: To explore the expression of NF-κB and HIF-1 in cervical intraepithelial neoplasia and cervical squamous cell carcinoma and to explore the clinicopathological significance. Methods: The cervical intraepithelial neoplasia and cervical squamous cell carcinoma cases were studied by immunohistochemical method to detect the expression of NF-κB and HIF-1 and to analyze the significance of its expression in cervical carcinogenesis. Results: The positive rates of NF-κB in normal cervical epithelium, CINⅠ, CINⅡ, CINⅢ and squamous cell carcinoma were 16%, 36%, 44%, 68% and 72% respectively. Overall there is a significant difference x2 = 21.636, p <0.01. Comparison between groups showed that there was a significant difference between normal epithelium and CIN Ⅲ and squamous cell carcinoma. The positive rates of HIF-1αin normal cervical epithelium, CINⅠ, CINⅡ, CINⅢ and squamous cell carcinoma were 12%, 20%, 40%, 68% and 76% respectively. The overall comparison there is a significant difference x2 = 32.733, p <0.01. A pairwise comparisons between groups showed significant difference between normal epithelium and CIN III (x2 = 16.333 p <0.001) and squamous cell carcinoma (x2 = 20.779 p <0.001). Conclusion: The expressions of NF-κB and HIF-1 are correlated with the occurrence of cervical squamous cell carcinoma and may be used as markers for early diagnosis.