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4-香豆酸:辅酶A连接酶(4CL)基因是植物调控木质素代谢、参与类黄酮和其他次生代谢产物合成的关键基因之一,而木质素的合成及聚合在细胞壁沉积导致部分薄壁细胞次生壁加厚形成石细胞。为更好了解砀山酥梨中4CL基因的种类和数量,本文利用砀山酥梨基因组的氨基酸和c DNA数据库对4CL基因家族进行筛选,分析了砀山酥梨基因组中4CL基因的种类、进化关系、物理定位、以及基因结构和保守基序。结果显示在砀山酥梨基因组中发现并初步确定了29个4CL基因,通过基因的定位分析发现除了4、8、11、12号染色体上没有4CL基因之外,其他染色体上都存在4CL基因;并且在9、17号染色体上还存在着基因簇。通过基因结构和进化树之间的比较,进一步确定了基因结构和进化之间的相互联系。研究结果为砀山酥梨4CL基因功能的深入分析奠定了基础。
4-Coumarate: Coenzyme A ligase (4CL) is one of the key genes in plant regulation of lignin metabolism, involved in the synthesis of flavonoids and other secondary metabolites, while the synthesis and polymerization of lignin results in partial thinning of the cell wall The secondary walls of parietal cells thickened to form stone cells. In order to understand the type and quantity of 4CL gene in Dangshan Pear better, 4CL gene family was screened by amino acid and c DNA database of Dangshansu pear genome. The species, evolutionary relationship, physical Location, and gene structure and conserved motifs. The results showed that 29 4CL genes were found and initially identified in the genome of Dangshansu pear. Based on the gene localization analysis, 4CL gene was found on all other chromosomes except 4, 4, 11, and 12 chromosomes; and There are also gene clusters on chromosomes 9 and 17. The comparison between gene structure and evolutionary tree further confirmed the interrelationship between gene structure and evolution. The results laid the foundation for further analysis of 4CL gene function in Dangshan Pear.