The increase of phosphorus concentration is a crucial factor causing the eutrophication of water body, while land use has an important impact on agricultural non-point sources (NPS) phosphorus discharge. Seven sites controlling the water in four sub-watersheds and the main exit of the Meicun Watershed of Xuancheng County, Anhui Province, were investigated by dynamic monitoring of stream water and nutrient discharge, integrating interpretation of aerial image and GIS analysis to find out how the land use affects phosphorus loss with stream water in typical agriculture-forest watershed in subtropical China. These monitored sites are different in structure of land use. Phosphorus concentration of the stream water was analyzed every week and at the next day of rainfall. The velocity of flow was measured by kinemometer to calculate the runoff flux and phosphorus discharge. The results showed that the runoff flux and the discharges of dissolved phosphorus (DP), particle-associated phosphorus (PAP) and total p The increase of phosphorus concentration is a crucial factor causing the eutrophication of water body, while land use has an important impact on agricultural non-point sources (NPS) phosphorus discharge. Seven sites controlling the water in four sub-watersheds and the main exit of the Meicun Watershed of Xuancheng County, Anhui Province, were investigated by dynamic monitoring of stream water and nutrient discharge, integrating interpretation of aerial image and GIS analysis to find out how the land use use phosphorus loss with stream water in typical agriculture-forest watershed in The monitored sites are different in structure of land use. Phosphorus concentration of the stream water was measured every week and at the next day of rainfall. The velocity of flow was measured by kinemometer to calculate the runoff flux and phosphorus discharge. results showed that the runoff flux and the discharges of dissolved phosphorus (DP), particle-associated phosphorus (PAP) and total p