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哈佛的基因研究所发现了两种特别有希望成为药物的集落刺激因子(CSF)。该公司采用Steve Clark发明的“表达克隆法”,找到了这些蛋白质的基因。研究人员从已知的人T细胞起作用的基因获取数百万个DNA片段,并用遗传工程将它们植入10万细菌菌落中,然后在实验室选择出似乎最能刺激细胞的那些片段。发现的一种片段叫做GM-CSF蛋白质基因,能刺激红细胞和两种白细胞(粒细胞和单核细胞)。该公司已将此基因克隆并转让给瑞士的山道士制药公司,用以制造试验用的药物。
Harvard’s Gene Institute has identified two particularly promising drug-like colony stimulating factors (CSFs). The company uses the “expression cloning” method invented by Steve Clark to find the genes for these proteins. The researchers took millions of DNA fragments from known genes that work on human T cells and genetically engineered them into 100,000 bacterial colonies and then picked up in the laboratory those fragments that seemed to stimulate the cells most. One of the found fragments, called the GM-CSF protein gene, stimulates red blood cells and two types of white blood cells (granulocytes and monocytes). The company has cloned and transferred the gene to Sandoz Pharmaceutical Company in Switzerland for the manufacture of a test drug.