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AIM: To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in downregulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS: The expression of survivin mRNA was measured by RTPCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60/ADR cells to adriamycin (ADR) was measured by MTT assay. RESULTS: pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60/ADR cells in a time-dependent manner during 12-48 h. After transient transfection with pcDNA3.1/survivin for 48 h, survivin mRNA decreased by 67％, survivin protein decreased by 57％, caspase-3 activity increased 4.37 times, and the apoptosis rate increased by 4.41％ compared wi
AIM: To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1 / survivin in downregulating the expression level of survivin mRNA and survivin protein with survivin protein and survivin protein reversed (MDR) in adriamycin-resistant HL-60 / ADR cell line. METHODS: The expression of survivin mRNA was measured by RTPCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60 / ADR cells RESULTS: The expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60 / ADR cells in a time-dependent manner during 12 -48 h. After transient transfection with pcDNA3.1 / survivin for 48 h, survivin mRNA decreased by 67%, survivin protein decreased by 57%, caspase-3 activity increased 4.37 times, and the apoptosis rate increased b y 4.41% compared wi