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目的:就电针预处理对大鼠局灶性脑缺血的影响进行实验研究。方法:采用SD大鼠(体质量280～340g,39只)作为研究对象,电针预处理大鼠百会、大椎穴,电针时间分别为15,30,60,90,120min,间隔1h后,造成局灶性脑缺血。行苏木精-伊红和末端脱氧核苷酸转移酶(TdT)介导的脱氧尿苷三磷酸(dUTP)切口末端标记(TUNEL)染色,测定大鼠脑梗死灶面积百分比和脑细胞凋亡情况。结果:电针预处理组中除15min组外,大鼠脑梗死灶面积百分比都比缺血对照组减小,脑细胞凋亡数也减少。预处理60min组脑梗死灶面积百分比和18700.2μm2凋亡细胞数分别为(27.8±4.2)%和(20.2±3.0)个,与缺血对照组犤(41.0±7.6)%和(31.0±4.4)个犦比较差异有显著性意义(P<0.05)。结论:电针预处理能够保护随后的缺血性脑损伤,且预处理需要达到一定的时间才具有保护作用。
Objective: To study the effect of electroacupuncture pretreatment on focal cerebral ischemia in rats. Methods: SD rats (body weight 280 ~ 340g, 39) were used as research objects. The electroacupuncture preconditioning was performed on Baihui and Dazhui acupoints for 15, 30, 60, 90 and 120 minutes respectively, Causes focal cerebral ischemia. Hematoxylin and eosin and terminal deoxynucleotidyl transferase (TdT) -mediated dUTP nick end labeling (TUNEL) staining were performed to determine the infarct size and brain cell apoptosis in rats Happening. Results: In acupuncture preconditioning group, except for the 15 min group, the area percentage of cerebral infarction lesions in rats decreased compared with the ischemic control group and the number of apoptotic cells decreased. The percentage of infarct size and the number of apoptotic cells of 18700.2μm2 in preconditioning 60min group were (27.8 ± 4.2)% and (20.2 ± 3.0), respectively, which were significantly lower than those in ischemic control group (41.0 ± 7.6% vs 31.0 ± 4.4% There was significant difference between each group (P <0.05). Conclusion: Electroacupuncture pretreatment can protect the subsequent ischemic brain injury, and pretreatment need to reach a certain time to have a protective effect.