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试验表明,病毒初次侵染来源于菌种,因此对带毒菌种进行脱毒处理,生产无毒菌种,杜绝病毒初次侵染来源,是防治平菇病毒病的关键措施。本试验,对带毒菌株通过在平板上切取菌落最外缘的尖端3~4mm幼嫩菌丝,经高温32~34℃培养,连续4次处理,对平菇菌种脱毒效果明显,病毒典型症状消失,子实体原基出现提早3~8天,产量提高15.1%~17.4%。 一、材料与方法 (一)供试带毒菌株 P56系从症状表现为菌盖小而畸形,边缘具深缺刻、波浪形、柄曲、表面凹凸不平的子实体,进行单菇组织分离而得。P59从症状表现为菌盖出现水渍状、浓淡不匀、对光可见透明条斑的单菇组
Experiments show that the primary infection of the virus originates from the strain, therefore, it is a key measure to prevent and treat Pleurotus ostreatus virus that the detoxification of the infected species, the production of non-toxic species, and the elimination of the primary source of virus infection. In this experiment, the infected strains were cut into 3 ~ 4mm young hyphae at the outermost edge of the colony on the plate and cultured at high temperature of 32 ~ 34 ℃ for 4 times. The detoxification effect on Pleurotus ostreatus was obvious. The virus Typical symptoms disappear, the fruiting body primordium appears earlier 3 to 8 days, the yield increased 15.1% ~ 17.4%. First, the material and methods (A) for the test with virulent strains P56 from the symptoms showed small cap deformity, edge with deep notch, wavy, handle song, uneven surface of the fruiting body, isolated from single mushroom tissue . P59 from the symptoms manifested as water cap-like appearance of cap, uneven shades of light visible transparent Monotone group