从新生小牛的胸主动脉分离血管内皮细胞 ,经形态学和Ⅷ因子相关抗原免疫染色鉴定 ,将其培养至一定数量 ;使用逆转录病毒载体介导的基因转移技术 ,通过病毒包装细胞 ,将人ｔＰＡ (组织型纤溶酶原激活剂 )ｃＤＮＡ导入内皮细胞中 ,分别用纤维蛋白板法和发色底物法检测到转基因内 Vascular endothelial cells were isolated from the thoracic aorta of neonatal calves and identified by morphological and factor Ⅷ-related antigen immunostaining and cultured to a certain amount. Using retroviral vector-mediated gene transfer technology, Human tPA (tissue-type plasminogen activator) cDNA was introduced into endothelial cells and detected within the transgene using the fibrin plate method and the chromogenic substrate method