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目的:探讨肢体缺血再灌注损伤对大鼠脊髓神经元Fos、一氧化氮合酶(NOS)、热休克蛋白70(HSP70)表达和分布的影响。方法:通过暂时阻断左侧髂总动脉和股动脉,建立肢体缺血再灌注损伤(IRI)模型。采用免疫组织化学ABC法,观察大鼠脊髓神经元Fos、NOS和HSP70阳性神经元的表达和分布特点。结果:大鼠肢体缺血4h,脊髓相应节段神经元Fos、NOS、HSP70表达增强(P<0.01),再灌注2h后,Fos、NOS、HSP70表达进一步增强(P<0.05),并且Fos、NOS、HSP70阳性神经元较集中分布于脊髓相应节段同侧后角及中央管周围(P<0.01)。结论:脊髓相应节段同侧神经元可能参与肢体IRI的发病过程,尤其是脊髓后角及中央管周围神经元可能具有更为重要的作用。
Objective: To investigate the effect of limb ischemia-reperfusion injury on the expression and distribution of Fos, nitric oxide synthase (NOS) and heat shock protein 70 (HSP70) in the spinal cord of rats. Methods: The model of limb ischemia-reperfusion injury (IRI) was established by temporarily blocking the common iliac artery and femoral artery. Immunohistochemical ABC method was used to observe the expression and distribution of Fos, NOS and HSP70 positive neurons in rat spinal cord neurons. Results: The expression of Fos, NOS and HSP70 were increased in the corresponding segments of the spinal cord 4 h after ischemia (P <0.01), and the expression of Fos, NOS and HSP70 were further increased 2 h after reperfusion (P <0.05) NOS and HSP70 positive neurons were concentrated in the ipsilateral posterior horn of the corresponding segment of the spinal cord and around the central canal (P <0.01). CONCLUSION: The ipsilateral neurons in the corresponding segments of the spinal cord may be involved in the pathogenesis of limb IRI, especially the spinal dorsal horn and the peripheral neurons in the central canal may play a more important role.