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目的:观察氧化苦参碱(oxymatrine,OMT)对骨髓来源细胞增殖的影响。方法:应用MTT比色法、流式细胞仪检测法、集落形成法和免疫细胞活性测定等方法,检测OMT对白血病细胞K562的抑制作用;骨髓造血干细胞的集落形成实验和脾细胞对肿瘤细胞的杀伤的生物活性试验,检测OMT对小鼠免疫和造血的影响。结果:(1)不同浓度的OMT可明显抑制白血病细胞K562细胞的增殖、集落形成,导致细胞凋亡(P<0.05),且作用呈浓度依赖性。(2)OMT可以促进小鼠骨髓粒系造血,且在0.2475 mg/mL时达到峰值。(3)OMT可抑制小鼠的免疫功能。结论:OMT可抑制白血病K562细胞的增殖并诱导其凋亡,同时抑制小鼠的免疫功能,促进小鼠骨髓CFU-GM的形成,表现出对骨髓来源细胞生长的双向调节作用。
Objective: To observe the effect of oxymatrine (OMT) on the proliferation of bone marrow-derived cells. Methods: The inhibitory effect of OMT on K562 leukemia cells was detected by MTT colorimetric assay, flow cytometry, colony formation assay and immunocyte activity assay. The bone marrow hematopoietic stem cell colony formation assay and the effect of splenocytes on tumor cells The biological activity of killing test to detect the impact of OMT immune and hematopoietic mice. Results: (1) OMT at different concentrations significantly inhibited the proliferation and colony formation of K562 leukemia cells, leading to apoptosis (P <0.05), and the effect was dose-dependent. (2) OMT can promote the myeloid granulocyte hematopoiesis in mice and reach its peak at 0.2475 mg / mL. (3) OMT can inhibit the immune function of mice. CONCLUSION: OMT can inhibit the proliferation and induce the apoptosis of K562 leukemia cells, inhibit the immune function of mice, promote the formation of CFU-GM in bone marrow of mice, and exhibit bidirectional regulation on the growth of bone marrow-derived cells.