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目的:非小细胞肺癌发生、发展的分子机制仍是目前研究的热点与难点,新近研究表明microRNA在肿瘤的发展过程中起着重要的作用。本研究旨在探讨miR-196a在人非小细胞肺癌组织及细胞系中的表达水平,以及抑制miR-196a对非小细胞肺癌细胞侵袭转移能力的影响。方法:通过real-time PCR技术检测人非小细胞肺癌及细胞系中miR-196a的表达水平,通过转染miR-196a inhibitors抑制miR-196a的表达水平,并通过定量PCR检测转染效率。利用transwell实验检测下调miR-196a对NCI-H1299细胞的迁移、侵袭能力的影响。结果:相对于正常肺组织及细胞,在非小细胞肺癌组织和细胞中miR-196a的表达水平出现了显著的上调,NCI-H1299细胞中转染miR-196a inhibitors能显著抑制miR-196a的表达水平且抑制miR-196a的表达能降低NCI-H1299细胞的迁移、侵袭能力。结论:定量PCR结果显示miR-196a在非小细胞肺癌组织及细胞中表达显著上调,而封闭其表达能影响非小细胞肺癌细胞的迁移和侵袭功能,提示miR-196a的表达上调可能在非小细胞肺癌的发生、发展中起着关键的作用,并有可能作为将来非小细胞肺癌诊断、预后的分子靶标。
OBJECTIVE: The molecular mechanism of the development and progression of non-small cell lung cancer is still the hot and difficult point of current research. Recent studies show that microRNA plays an important role in the development of tumors. The purpose of this study was to investigate the expression of miR-196a in human non-small cell lung cancer tissues and cell lines, as well as the effect of miR-196a on invasion and metastasis of non-small cell lung cancer cells. Methods: The expression of miR-196a in human non-small cell lung cancer and cell lines was detected by real-time PCR. The miR-196a expression was inhibited by transfection with miR-196a inhibitors and the transfection efficiency was detected by quantitative PCR. Transwell assay was used to detect the effect of miR-196a on the migration and invasion of NCI-H1299 cells. Results: Compared with normal lung tissues and cells, miR-196a expression was significantly up-regulated in non-small cell lung cancer tissues and cells. MiR-196a inhibitors transfected NCI-H1299 cells significantly inhibited the expression of miR-196a The level of miR-196a can inhibit the migration and invasion of NCI-H1299 cells. Conclusion: The results of quantitative PCR showed that the expression of miR-196a was significantly up-regulated in non-small cell lung cancer tissues and cells, whereas the blockade of miR-196a expression could affect the migration and invasion of non-small cell lung cancer cells. The occurrence and development of cell lung cancer play a key role and may serve as molecular targets for the diagnosis and prognosis of non-small cell lung cancer in the future.