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目的 探究典型的外源性雌激素对 -壬基酚 (p- NP)的幼雌 SD大鼠子宫营养试验敏感指标及其机理。方法 用 2 1d龄 SD雌性大鼠 5 0只 ,随机分为花生油溶剂对照组 ,p- NP6 0 mg/ kg、90 mg/ kg和 12 0 mg/ kg,苯甲酸雌二醇 (E2 B,0 .4 mg/ kg)阳性对照组共 5组 ,每天灌胃一次给药 ,连续 3d。于末次给药后 2 4 h处死动物 ,取子宫称湿质量。用免疫组化技术检测子宫雌激素基因产物孕激素受体 (PR)、雌激素受体 (ER)和增殖细胞核抗原(PCNA)表达情况。结果 E2 B0 .4 m g/ kg、p- NP90 mg/ kg和 12 0 m g/ kg组的子宫湿质量、子宫 /体质量比与溶剂对照组相比 ,均有显著性差异 (P<0 .0 1) ,且有明显的剂量效应关系。而 p- NP6 0 mg/ kg组的子宫固有层和肌层细胞核有 PR,ER及 PCNA表达 ,且有随剂量递增趋势。结论 子宫营养试验检测雌激素基因产物比其它指标敏感 ,固有层和肌层细胞增生是子宫湿质量增加的原因。
Objective To investigate the sensitive index and mechanism of uterus nutrition test in young SD rats treated with p-NP, a typical exogenous estrogen. Methods Fifty SD female SD rats were randomly divided into peanut oil control group, p-NP6 0 mg / kg, 90 mg / kg and 120 mg / kg estradiol benzoate (E2 B, 0 .4 mg / kg) positive control group a total of 5 groups, once a day by intragastric administration, continuous 3d. Animals were sacrificed 24 h after the last administration, and the uterus was called wet weight. The expression of progesterone receptor (PR), estrogen receptor (ER) and proliferating cell nuclear antigen (PCNA) in uterine estrogen were detected by immunohistochemistry. Results Compared with the solvent control group, the uterine wet mass and the uterine mass ratio of E2 B0.4 mg / kg, p-NP90 mg / kg and 120 mg / kg groups were significantly different (P <0. 0 1), and there is a clear dose-response relationship. However, the expressions of PR, ER and PCNA in lamina propria and muscle nucleus in p-NP6 0 mg / kg group increased with dose. Conclusion Uterine nutrition test detected estrogen gene products than other indicators of sensitivity, lamina propria and myometrial cell proliferation is the cause of increased uterine wet mass.