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[目的]筛选出适宜玉米成熟胚组织培养的基因型和基本培养基。[方法]以9个基因型的玉米成熟胚为外植体,研究基因型与基本培养基对成熟胚愈伤组织诱导和继代的影响。[结果]试验中表现较好的基因型依次是853-35、853-209、丹34和81162;对于玉米成熟胚愈伤组织的诱导培养,MS培养基比N6培养基更适宜,而对于玉米成熟胚愈伤组织的继代培养,N6的培养基比MS培养基更适宜。[结论]进一步完善以玉米成熟胚为外植体的组织培养体系。
[Objective] The research aimed to select the suitable genotypes and basic culture medium for maize embryonic tissue culture. [Method] Nine mature genotypes of maize embryos were used as explants to study the effects of genotype and minimal medium on callus induction and subculture of mature embryos. [Result] The genotypes which performed better in the experiment were 853-35, 853-209, Dan 34 and 81162, respectively. MS medium was more suitable than the N6 medium for induction culture of maize embryo callus, Subculture of mature embryo callus, N6 medium is more suitable than MS medium. [Conclusion] The tissue culture system with mature maize embryo as explant was further perfected.