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目的探讨基质金属蛋白酶9(MMP9)及其组织抑制物(TIMP1)在人结核性淋巴结中的表达及其临床意义。方法免疫组化SP法检测MMP9和TIMP1蛋白在20例活动性淋巴结结核、20例陈旧性结核性淋巴结病灶和20例慢性非特异性淋巴结炎中的表达,采用图象分析技术对免疫组化结果进行定量分析。结果MMP9和TIMP1在三组淋巴结病变均有表达,其中MMP9在活动性淋巴结结核的肉芽肿、坏死周围炎性区呈强表达,在非特异性淋巴结炎和陈旧性结核病灶呈弱表达。MMP9,TIMP1及MMP9/TIMP1在活动性结核组表达显著高于非特异性淋巴结炎和陈旧性结核病灶(均为P<0.01),在单纯活动性淋巴结核组和合并活动性肺结核组表达无显著性差异(均为P>0.05)。结论MMP9、TIMP1高表达可能在结核性淋巴结炎发病机制中发挥重要作用,MMP9/TIMP1失衡可能预示疾病进展和结核播散。
Objective To investigate the expression and clinical significance of matrix metalloproteinase 9 (MMP9) and its tissue inhibitor of metalloproteinase (TIMP1) in human tuberculous lymph nodes. Methods Immunohistochemical SP method was used to detect the expression of MMP9 and TIMP1 in 20 cases of active lymph node tuberculosis, 20 cases of old tuberculous lymph node lesions and 20 cases of chronic non-specific lymphadenitis. The results of immunohistochemistry were analyzed by image analysis Quantitative analysis. Results MMP9 and TIMP1 were all expressed in all three groups of lymph node lesions. Among them, MMP9 was strongly expressed in granulomas of active lymph node tuberculosis and inflammatory areas around necrosis and weakly in nonspecific lymphadenitis and old tuberculosis lesions. The expressions of MMP9, TIMP1 and MMP9 / TIMP1 in active tuberculosis group were significantly higher than those in non-specific lymphadenitis and old tuberculosis (all P <0.01), but not in active lymph node group and active tuberculosis group Difference (all P> 0.05). Conclusion The high expression of MMP9 and TIMP1 may play an important role in the pathogenesis of tuberculous lymphadenitis. The imbalance of MMP9 / TIMP1 may predict the progression of disease and the spread of tuberculosis.